Search results for "Clinical Laboratories"

showing 5 items of 5 documents

Impact of Pre-Analytical Time on the Recovery of Pathogens from Blood Cultures: Results from a Large Retrospective Survey.

2017

Background Prompt identification of bloodstream pathogens is essential for optimal management of patients. Significant changes in analytical methods have improved the turnaround time for laboratory diagnosis. Less attention has been paid to the time elapsing from blood collection to incubation and to its potential effect on recovery of pathogens. We evaluated the performance of blood cultures collected under typical hospital conditions in relation to the length of their pre-analytical time. Methods We carried out a large retrospective study including 50,955 blood cultures collected, over a 30-month period, from 7,035 adult septic patients. Cultures were accepted by the laboratory only durin…

0301 basic medicineMaleTime FactorsResearch FacilitiesPhysiologylcsh:MedicineBacteremiaYeast and Fungal ModelsMedicine (all); Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)Pathology and Laboratory MedicineTurnaround timeSurveys and QuestionnairesMedicine and Health SciencesMedicineBlood culturelcsh:ScienceIncubationCandidaFungal PathogensMultidisciplinarymedicine.diagnostic_testPre analyticalHematologyMiddle AgedHospitalsClinical Laboratory SciencesBody FluidsClinical LaboratoriesBloodItalyExperimental Organism SystemsMedical MicrobiologyFemaleAnatomyPathogensResearch LaboratoriesResearch ArticleBiotechnologymedicine.medical_specialtyCatheters030106 microbiologyMycologyResearch and Analysis MethodsMicrobiologySpecimen HandlingSepsis03 medical and health sciencesSigns and SymptomsRetrospective surveyDiagnostic MedicineInternal medicineSepsisHumansCandida AlbicansMicrobial PathogensAgedRetrospective StudiesBacteriological TechniquesBacteriabusiness.industryClinical Laboratory Techniqueslcsh:ROrganismsFungiBiology and Life SciencesRetrospective cohort studymedicine.diseaseYeastSurgeryBlood CultureBacteremialcsh:QMedical Devices and EquipmentbusinessGovernment LaboratoriesPloS one
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Anisakis sensitization in different population groups and public health impact. A systematic review

2018

Anisakis simplex spp. sensitization rates have increased worldwide, with a significant impact on health-care systems. To date, no clear-cut diagnostic criteria and laboratory algorithm have been established, so anisakiasis still represents an under-reported health problem whose clinical manifestations, when present, mimic the much more common allergic and digestive disorders. Aim of the study was to systematically review the available literature on the prevalence of sensitization against Anisakis in the general population and in specific population groups, taking into account the impact of the different available diagnostic techniques on the epidemiological data. Following the Preferred Rep…

0301 basic medicineNematodaUrticarialcsh:MedicineSettore MED/42 - Igiene Generale E ApplicataAnisakisGeographical locations0302 clinical medicineAllergiesEpidemiologyMedicine and Health SciencesantibodiesEnzyme-Linked Immunoassayslcsh:SciencehumanshelminthSensitizationeducation.field_of_studyMultidisciplinaryAllergic DiseasesbiologyShellfish allergyEukaryotaanimals; anisakis; antibodies helminth; enzyme-linked immunosorbent assay; humans; hypersensitivity; occupational exposureClinical Laboratory SciencesEuropeanimalsClinical Laboratoriesmedicine.anatomical_structureSystematic reviewhypersensitivityResearch Articlemedicine.medical_specialtyImmunologyImmunoblotting030231 tropical medicinePopulationFood AllergiesAntibodies HelminthMolecular Probe TechniquesDermatologyResearch and Analysis Methods03 medical and health sciencesDiagnostic MedicineEnvironmental healthmedicineEuropean UnionImmunoassaysMolecular Biology TechniqueseducationMolecular BiologyBiochemistry Genetics and Molecular Biology (all)business.industryPublic healthlcsh:RAnisakis simplexOrganismsBiology and Life Sciencesoccupational exposureanisakismedicine.diseasebiology.organism_classificationInvertebrates030104 developmental biologyAgricultural and Biological Sciences (all)SpainImmunologic TechniquesClinical Immunologylcsh:Qenzyme-linked immunosorbent assayClinical MedicinePeople and placesbusiness
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Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.

2017

We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratorie…

0301 basic medicinePhysiologyMicroorganismlcsh:MedicinePathology and Laboratory MedicineNervous SystemMass SpectrometryAnalytical Chemistrychemistry.chemical_compoundSpectrum Analysis TechniquesMicrobial PhysiologyCandida albicansMedicine and Health SciencesMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometrylcsh:ScienceCerebrospinal FluidMultidisciplinaryChemistryMicrobial Growth and DevelopmentClinical Laboratory SciencesBody FluidsClinical microbiologyChemistryClinical LaboratoriesThioglycolatesPhysical SciencesAnaerobic bacteriaAnatomyCellular DebrisResearch ArticleClinical PathologyThioglycolate broth030106 microbiologyAnaerobic BacteriaResearch and Analysis MethodsMicrobiology03 medical and health sciencesSigns and SymptomsDiagnostic MedicineGram Negative BacteriaLaboratory methodsChromatographyBacteriaBacterial Growthlcsh:ROrganismsBiology and Life SciencesBacteriologyCulture MediaMatrix-assisted laser desorption/ionizationAbscessesClinical MicrobiologySpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationlcsh:QLaboratoriesDevelopmental BiologyPLoS ONE
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Performance of two HCV RNA assays during protease inhibitor-based triple therapy in patients with advanced liver fibrosis and cirrhosis.

2014

Introduction: On-treatment HCV RNA measurements are crucial for the prediction of a sustained virological response (SVR) and to determine treatment futility during protease inhibitor-based triple therapies. In patients with advanced liver disease an accurate risk/benefit calculation based on reliable HCV RNA results can reduce the number of adverse events. However, the different available HCV RNA assays vary in their diagnostic performance. Aim: To investigate the clinical relevance of concordant and discordant results of two HCV RNA assays during triple therapy with boceprevir and telaprevir in patients with advanced liver fibrosis/cirrhosis. Methods: We collected on-treatment samples of 1…

Liver CirrhosisViral DiseasesCirrhosisGastroenterology and hepatologyHepaciviruslcsh:MedicineHepacivirusmedicine.disease_causeGastroenterologyTelaprevirHepatitisLiver diseasechemistry.chemical_compoundMedicinelcsh:ScienceMultidisciplinarybiologyvirus diseasesHepatitis CHepatitis CClinical Laboratory SciencesEuropeClinical LaboratoriesInfectious hepatitisInfectious DiseasesTreatment OutcomeAnti-Retroviral AgentsHCVRNA ViralOligopeptidesmedicine.drugResearch Articlemedicine.medical_specialtyGenotypeProlineHepatitis C virusDiagnostic MedicinePredictive Value of TestsBoceprevirInternal medicineHumansProtease InhibitorsViremiaddc:610Liver diseasesMedicine and health sciencesbusiness.industryClinical Laboratory Techniqueslcsh:RRNAReproducibility of Resultsmedicine.diseasebiology.organism_classificationdigestive system diseaseschemistryImmunologylcsh:Qbusiness
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Développement de méthodes de référence pour les biomarqueurs du bilan lipidique : application au contrôle qualité en biologie clinique

2013

Reliable measurements in medical biology are essential for early screening and appropriate follow-up of patients. Ensuring metrological traceability of clinical measurements to higher order reference methods or certified reference materials enables to obtain comparable results over time and between different laboratories that could use different methods to quantify the same biomarker.In this study, reference methods were developed and validated for lipid profile biomarkers (total cholesterol, triglycerides, HDL-C, and LDL-C). Their value added in proficiency testing schemes was demonstrated against consensus mean. They were also used to characterize a certified reference material (CRM) that…

[SDV.SA]Life Sciences [q-bio]/Agricultural sciencesMetrological traceability[SDV.SA] Life Sciences [q-bio]/Agricultural sciencesMass spectrometryCommutabilityProficiency testing schemesSpectrométrie de masseLaboratoires de biologie médicaleMéthodes de référenceClinical LaboratoriesCholesterolReference methodsCholestérolCommutabilitéCertified Reference MaterialsTraçabilité métrologiqueEvaluation externe de la qualitéTriglycéridesMatériaux de Référence Certifiés[ SDV.SA ] Life Sciences [q-bio]/Agricultural sciencesTriglycerides
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